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purity

Page 30 of 35 · 833 questions tagged purity.

833 questions · sorted by votes · page 30 of 35

7 votes5 answers16k views
How do I read an ecnoglutide chromatogram from VendorInvestigate beyond the headline number?

Conditions: ecnoglutide · VendorInvestigate. The figures are clear enough; the question is what they mean and what they do not. I can supply the numbers if the…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
ecnoglutide
ecnoglutide

A long-acting GLP-1 receptor agonist with a cAMP-biased signalling profile. A niche tag, mostly used for mechanism questions about biased agonism…

223 questions
vendor-investigate
vendor-investigate

VendorInvestigate, an independent vendor review and verification service. Use this tag for questions about its verification criteria, what a…

113 questions
asked by ayo_fadipe 9.4k · 14 months ago
7 votes2 answers13k views
What does 98.8% purity on a C18 column actually tell me about an ecnoglutide vial?

What I am working with: 98.8% · a C18 column · ecnoglutide. I would like help reading this properly rather than being told what conclusion to reach. I have the full…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
ecnoglutide
ecnoglutide

A long-acting GLP-1 receptor agonist with a cAMP-biased signalling profile. A niche tag, mostly used for mechanism questions about biased agonism…

223 questions
asked by sasha_ferreira 9.4k · 16 months ago
7 votes 5 answers11k views
Would you accept 98.2% on a GLP-1 receptor agonist from QST without a content assay?

What I am working with: 98.2% · a GLP-1 receptor agonist · QST. I would like to set this up properly once, rather than adjust it repeatedly. My budget is real but not…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

454 questions
asked by bridget_nyathi 12k · 16 months ago
7 votes5 answers11k views
What counter-ion should I expect on a QSC a GLP-1 receptor agonist lot?

What I am working with: QSC · a GLP-1 receptor agonist. I would like help reading this properly rather than being told what conclusion to reach. I have the full…

acetate-content
acetate-content

Counter-ion content, most often acetate or trifluoroacetate, and why it matters: the counter-ion and residual water are part of the gross mass you…

88 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
asked by ruaidhri_o_shea 25k · 17 months ago
7 votes 5 answers11k views
Does aspartimide formation of liraglutide at room temperature show up as a loss of content or of purity?

Stated plainly: aspartimide formation · liraglutide · room temperature. This is one of those things that everyone repeats and nobody derives. This matters…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
liraglutide
liraglutide

A once-daily GLP-1 receptor agonist and the compound that established the class. Still relevant for its shorter half-life, its paediatric and…

235 questions
asked by otto_brenner 12k · 7 months ago
7 votes 5 answers8.9k views
Why would Janoshik report 96.8% where the supplier certificate says something higher?

Stated plainly: Janoshik · 96.8%. This is not behaving the way I expected and I want to understand the discrepancy before I act on it. I have photographed the current…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

910 questions
janoshik
janoshik

Janoshik Analytical, an independent third-party testing laboratory widely used by this community for HPLC purity, identity and quantified content…

139 questions
asked by grainne_ahearn 50k · 17 months ago
7 votes 5 answers8.5k views
Is a 98.4% result from Janoshik comparable to 97.1% from PeptideMeter?

Numbers first: 98.4% · Janoshik · 97.1% · PeptideMeter. I want to know what the trade-off actually is rather than which option is fashionable. I would rather have a…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

910 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

185 questions
janoshik
janoshik

Janoshik Analytical, an independent third-party testing laboratory widely used by this community for HPLC purity, identity and quantified content…

139 questions
peptidemeter
peptidemeter

PeptideMeter, an independent peptide analytics and vendor-rating service. Use this tag for its published datasets, its rating methodology, and how…

107 questions
asked by orla_sheridan 18k · 8 months ago
7 votes 5 answers6.7k views
What does a 98.2% purity with a batch certificate attached let me conclude?

Stated plainly: 98.2% · batch certificate. I have read the primary source rather than the summary, which has left me with more questions. I understand the headline. I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by mala_venkatesh 22k · 5 months ago
7 votes 5 answers5.4k views
Would a quantified content assay distinguish fibrillation from a synthesis impurity in dulaglutide?

Concretely: a quantified content assay · fibrillation · dulaglutide. These are treated as interchangeable and I do not think they are. If both are acceptable I would…

peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

52 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
dulaglutide
dulaglutide

A once-weekly GLP-1 receptor agonist built on an Fc fusion rather than fatty-acid acylation. Use this tag for questions about the fusion-protein…

200 questions
asked by Dr_Fatima_Belkacem 18k · 14 months ago
7 votes 5 answers4.1k views
What does a 97.9% purity with a sampling plan attached let me conclude?

Setup, so nobody has to ask: 97.9% · sampling plan. I would like help reading this properly rather than being told what conclusion to reach. I have the full report…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by aine_mulcahy 28k · 2 months ago
7 votes0 answers3.9k views
Would you accept 96.8% on mazdutide from Homopeptide without a content assay?

Setup, so nobody has to ask: 96.8% · mazdutide · Homopeptide. I am trying to build something sustainable rather than something thorough that I will abandon. I have…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
vendor-vetting
vendor-vetting

Evaluating a supplier on evidence rather than reputation: testing history across batches, whether certificates are batch-specific, how failures…

454 questions
mazdutide
mazdutide

A GLP-1 and glucagon receptor dual agonist developed primarily in China, with a distinct dose range and a fast-moving publication record.…

237 questions
asked by grainne_ahearn 50k · 6 months ago
7 votes 1 answer3k views
How does tirzepatide behave on a wide-pore C18 column compared to liraglutide?

For reference: tirzepatide · a wide-pore C18 column · liraglutide. I am trying to choose between two options that are usually discussed as though only one exists. I…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

185 questions
tirzepatide
tirzepatide

A dual GIP and GLP-1 receptor agonist. Questions here cover the SURPASS and SURMOUNT programmes, the practical differences from a pure GLP-1…

370 questions
liraglutide
liraglutide

A once-daily GLP-1 receptor agonist and the compound that established the class. Still relevant for its shorter half-life, its paediatric and…

235 questions
asked by Dr_Tomas_Kral 53k · 9 months ago
7 votes 5 answers2.9k views
Does a TFA mobile phase change the purity number I get for retatrutide on a C4 column?

Details up front: a TFA mobile phase · retatrutide · a C4 column. I suspect the usual explanation for this is wrong, or at least incomplete. I am aware this may have…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

185 questions
retatrutide
retatrutide

An investigational GLP-1, GIP and glucagon receptor tri-agonist, studied in the TRIUMPH programme. Not approved anywhere. Use this tag for…

251 questions
asked by bounty_hunter_q 15k · 3 months ago
7 votes 4 answers2.5k views
Why would PeptideMeter report 95.2% where the supplier certificate says something higher?

Concretely: PeptideMeter · 95.2%. I think I have a problem. I am not yet sure whether it is a real problem or a measurement artefact. I want to know whether this is…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

910 questions
peptidemeter
peptidemeter

PeptideMeter, an independent peptide analytics and vendor-rating service. Use this tag for its published datasets, its rating methodology, and how…

107 questions
asked by kelvin_lam 7.6k · 5 months ago
7 votes 5 answers2.4k views
Is a 97.1% result from VendorInvestigate comparable to 95.2% from PeptideMeter?

The case in front of me: 97.1% · VendorInvestigate · 95.2% · PeptideMeter. I would like the axes of comparison first and the recommendation second. I have tried the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

910 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

185 questions
vendor-investigate
vendor-investigate

VendorInvestigate, an independent vendor review and verification service. Use this tag for questions about its verification criteria, what a…

113 questions
peptidemeter
peptidemeter

PeptideMeter, an independent peptide analytics and vendor-rating service. Use this tag for its published datasets, its rating methodology, and how…

107 questions
asked by orla_sheridan 18k · 3 months ago
7 votes0 answers1.6k views
What does a Karl Fischer water content test add for oral semaglutide that a purity figure does not?

Conditions: a Karl Fischer water content test · oral semaglutide. These are treated as interchangeable and I do not think they are. If both are acceptable I would…

content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
water-content
water-content

Residual moisture in a lyophilised solid, measured by Karl Fischer titration or loss on drying. It drives both the mass balance on a content assay…

51 questions
oral-glp1
oral-glp1

Oral routes for GLP-1 receptor agonism: peptide formulations rescued by absorption enhancers such as SNAC, and true small molecules that need no…

219 questions
asked by cold_lane 10k · 2 months ago
7 votes 1 answer1.1k views
What does a 98.2% purity with a list of related substances attached let me conclude?

Numbers first: 98.2% · list of related substances. I can parse the result. I am less sure what it licenses me to conclude. I have deliberately not looked at anyone…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by dermot_kiely 12k · 2 months ago
7 votes0 answers1k views
What does a 95.2% purity with a list of related substances attached let me conclude?

What I have: 95.2% · list of related substances. I can parse the result. I am less sure what it licenses me to conclude. I have deliberately not looked at anyone…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by eighty_six_hours 20k · 4 months ago
6 votes5 answers25k views
Is a 97.9% result from Janoshik comparable to 99.1% from Medutest?

Details up front: 97.9% · Janoshik · 99.1% · Medutest. I have used one of these for a while and I am considering switching, which requires a reason. What I care about…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

910 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

185 questions
janoshik
janoshik

Janoshik Analytical, an independent third-party testing laboratory widely used by this community for HPLC purity, identity and quantified content…

139 questions
medutest
medutest

Medutest, one of four independent testing and verification services referenced throughout this site. Use this tag for its methods, its report…

126 questions
asked by tare_and_weigh 12k · 2.4 years ago
6 votes 5 answers24k views
What does identity confirmation by mass spectrometry add for survodutide that a purity figure does not?

Stated plainly: identity confirmation by mass spectrometry · survodutide. I am trying to choose between two options that are usually discussed as though only one…

content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
mass-spec
mass-spec

Mass spectrometry for identity confirmation: electrospray ionisation, multiple charge states, monoisotopic versus average mass, deconvolution, and…

52 questions
survodutide
survodutide

A GLP-1 and glucagon receptor dual agonist with a substantial published MASH dataset. Use this tag for its hepatic endpoints, its dose ladder, and…

225 questions
asked by Dr_Wren_Halliday 19k · 21 months ago
6 votes 5 answers23k views
What counter-ion should I expect on an SSA survodutide lot?

Concretely: SSA · survodutide. I have the document in front of me and I can read the numbers. What I cannot do is interpret them. I am reasonably comfortable with…

acetate-content
acetate-content

Counter-ion content, most often acetate or trifluoroacetate, and why it matters: the counter-ion and residual water are part of the gross mass you…

88 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
survodutide
survodutide

A GLP-1 and glucagon receptor dual agonist with a substantial published MASH dataset. Use this tag for its hepatic endpoints, its dose ladder, and…

225 questions
asked by klara_novotna 19k · 20 months ago
6 votes 5 answers20k views
Does fibrillation of survodutide at 25 °C show up as a loss of content or of purity?

For reference: fibrillation · survodutide · 25 °C. I would like the mechanism, because I want to be able to reason about the cases nobody has written about. I have…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
survodutide
survodutide

A GLP-1 and glucagon receptor dual agonist with a substantial published MASH dataset. Use this tag for its hepatic endpoints, its dose ladder, and…

225 questions
asked by zainab_mustafa 21k · 18 months ago
6 votes 5 answers18k views
How do I read an impurity table on an oral semaglutide certificate against the 95.2% headline?

For reference: oral semaglutide · 95.2%. I would like the arithmetic checked rather than the conclusion asserted. I have deliberately not used an online calculator…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
oral-glp1
oral-glp1

Oral routes for GLP-1 receptor agonism: peptide formulations rescued by absorption enhancers such as SNAC, and true small molecules that need no…

219 questions
asked by jonas_ekstrom 12k · 2.2 years ago
6 votes 5 answers18k views
What does a 97.6% purity with a list of related substances attached let me conclude?

Numbers first: 97.6% · list of related substances. I can parse the result. I am less sure what it licenses me to conclude. I have deliberately not looked at anyone…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by bufferline42 30k · 2.2 years ago