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hplc

Page 11 of 21 · 503 questions tagged hplc.

503 questions · sorted by votes · page 11 of 21

19 votes0 answers23k views
Does hydrolysis dominate for retatrutide held at minus 80 °C?

Numbers first: hydrolysis · retatrutide · minus 80 °C. I can predict the outcome but I cannot explain it, which means I will get the next case wrong. I would like to…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

701 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
retatrutide
retatrutide

An investigational GLP-1, GIP and glucagon receptor tri-agonist, studied in the TRIUMPH programme. Not approved anywhere. Use this tag for…

251 questions
asked by yuki_morishita 10k · 2.1 years ago
19 votes 5 answers15k views
What does a 96.4% purity with a method section attached let me conclude?

Conditions: 96.4% · method section. The figures are clear enough; the question is what they mean and what they do not. I can supply the numbers if the specifics…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by g_paskevicius 60k · 11 months ago
19 votes 5 answers11k views
What does an HPLC purity test add for dulaglutide that a purity figure does not?

What I am working with: an HPLC purity test · dulaglutide. I have used one of these for a while and I am considering switching, which requires a reason. What I care…

content-assay
content-assay

Quantified content: how many milligrams of peptide are actually in the vial, measured against a calibrated reference standard. A separate test…

476 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
dulaglutide
dulaglutide

A once-weekly GLP-1 receptor agonist built on an Fc fusion rather than fatty-acid acylation. Use this tag for questions about the fusion-protein…

200 questions
asked by dermot_kiely 12k · 12 months ago
19 votes 2 answers8.5k views
How does oral semaglutide behave on a wide-pore C18 column compared to semaglutide?

The particulars: oral semaglutide · a wide-pore C18 column · semaglutide. The comparison I want does not seem to exist anywhere in a form I can evaluate. I have read…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
reference-standard
reference-standard

Certified reference materials and in-house standards: traceability, the certificate of the standard itself, system suitability, and why every…

185 questions
oral-glp1
oral-glp1

Oral routes for GLP-1 receptor agonism: peptide formulations rescued by absorption enhancers such as SNAC, and true small molecules that need no…

219 questions
semaglutide
semaglutide

A GLP-1 receptor agonist with a fatty-acid-acylated backbone and a roughly one-week half-life, marketed for type 2 diabetes and for weight…

470 questions
asked by Dr_Hanne_Solberg 36k · 3 months ago
19 votes0 answers7.4k views
What does a 97.6% purity with a batch certificate attached let me conclude?

Stated plainly: 97.6% · batch certificate. I have read the primary source rather than the summary, which has left me with more questions. I understand the headline. I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by pierce_count 24k · 4 months ago
19 votes 5 answers6.3k views
What does a 96.4% purity with a list of related substances attached let me conclude?

Details up front: 96.4% · list of related substances. I can parse the result. I am less sure what it licenses me to conclude. I have deliberately not looked at anyone…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by teodora_ilic 17k · 7 months ago
19 votes 5 answers5.1k views
What does 96.4% purity on a wide-pore C18 column actually tell me about a GLP-1 receptor agonist vial?

For reference: 96.4% · a wide-pore C18 column · a GLP-1 receptor agonist. I want to understand what this actually establishes, as opposed to what it is being used to…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
asked by bea_castellanos 24k · 4 months ago
19 votes 3 answers4.3k views
What does 97.6% purity on a wide-pore C18 column actually tell me about a mazdutide vial?

For reference: 97.6% · a wide-pore C18 column · mazdutide. I want to understand what this actually establishes, as opposed to what it is being used to imply. My…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
mazdutide
mazdutide

A GLP-1 and glucagon receptor dual agonist developed primarily in China, with a distinct dose range and a fast-moving publication record.…

237 questions
asked by marta_okonkwo 190k · 3 months ago
19 votes5 answers3.6k views
How do I read a survodutide chromatogram from VendorInvestigate beyond the headline number?

The case in front of me: survodutide · VendorInvestigate. The figures are clear enough; the question is what they mean and what they do not. I can supply the numbers…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
survodutide
survodutide

A GLP-1 and glucagon receptor dual agonist with a substantial published MASH dataset. Use this tag for its hepatic endpoints, its dose ladder, and…

225 questions
vendor-investigate
vendor-investigate

VendorInvestigate, an independent vendor review and verification service. Use this tag for questions about its verification criteria, what a…

113 questions
asked by helena_vidmar 8.5k · 16 days ago
18 votes 5 answers36k views
Is 96.4% a realistic purity ceiling for oral semaglutide given its chain length?

The case in front of me: 96.4% · oral semaglutide. Please show the division. I want to check my own against yours. I would like the general form as well as the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questions
oral-glp1
oral-glp1

Oral routes for GLP-1 receptor agonism: peptide formulations rescued by absorption enhancers such as SNAC, and true small molecules that need no…

219 questions
asked by one_ml_bac 18k · 2.3 years ago
18 votes 5 answers34k views
Does aggregation dominate for a GLP-1 receptor agonist held at 40 °C?

What I am working with: aggregation · a GLP-1 receptor agonist · 40 °C. I keep seeing this stated as a fact with no explanation attached, and unexplained facts make…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

701 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by t_oyelaran 79k · 21 months ago
18 votes 5 answers30k views
What does 99.4% purity on a C18 column actually tell me about a semaglutide vial?

Setup, so nobody has to ask: 99.4% · a C18 column · semaglutide. I would like help reading this properly rather than being told what conclusion to reach. I have the…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
semaglutide
semaglutide

A GLP-1 receptor agonist with a fatty-acid-acylated backbone and a roughly one-week half-life, marketed for type 2 diabetes and for weight…

470 questions
asked by samir_bennani 15k · 22 months ago
18 votes 5 answers28k views
Does hydrolysis produce a resolvable peak on a C8 column, or does it co-elute?

Details up front: hydrolysis · a C8 column. I can predict the outcome but I cannot explain it, which means I will get the next case wrong. I would like to know how…

hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
asked by fiadh_cronin 58k · 17 months ago
18 votes5 answers28k views
Why would Janoshik report 96.4% where the supplier certificate says something higher?

What I am working with: Janoshik · 96.4%. I noticed this today and I have not touched anything since, in case the state is diagnostic. I have not discarded anything…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

910 questions
janoshik
janoshik

Janoshik Analytical, an independent third-party testing laboratory widely used by this community for HPLC purity, identity and quantified content…

139 questions
asked by assay_blank 45k · 23 months ago
18 votes 5 answers28k views
Does aspartimide formation dominate for tirzepatide held at 2–8 °C?

Concretely: aspartimide formation · tirzepatide · 2–8 °C. I want to know whether this is a real physical effect or an artefact of how it is measured. What prompted…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

701 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
tirzepatide
tirzepatide

A dual GIP and GLP-1 receptor agonist. Questions here cover the SURPASS and SURMOUNT programmes, the practical differences from a pure GLP-1…

370 questions
asked by jonas_ekstrom 12k · 2.0 years ago
18 votes5 answers27k views
Is 98.2% a realistic purity ceiling for tirzepatide given its chain length?

Conditions: 98.2% · tirzepatide. Please show the division. I want to check my own against yours. I would like the general form as well as the specific number, so I…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questions
tirzepatide
tirzepatide

A dual GIP and GLP-1 receptor agonist. Questions here cover the SURPASS and SURMOUNT programmes, the practical differences from a pure GLP-1…

370 questions
asked by cake_collapsed 14k · 2.1 years ago
18 votes 5 answers24k views
Does aggregation dominate for mazdutide held at minus 80 °C?

Stated plainly: aggregation · mazdutide · minus 80 °C. I keep seeing this stated as a fact with no explanation attached, and unexplained facts make me suspicious. My…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

701 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
mazdutide
mazdutide

A GLP-1 and glucagon receptor dual agonist developed primarily in China, with a distinct dose range and a fast-moving publication record.…

237 questions
asked by b_delacroix 43k · 2.2 years ago
18 votes 5 answers24k views
What does a 98.8% purity with a batch certificate attached let me conclude?

What I am working with: 98.8% · batch certificate. I have read the primary source rather than the summary, which has left me with more questions. I understand the…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
coa
coa

Certificates of analysis: what fields a useful one carries, how to tell a real analytical report from a marketing document, batch and lot…

771 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
asked by two_two_micron 9.3k · 19 months ago
18 votes3 answers22k views
Is 98.4% a realistic purity ceiling for tirzepatide given its chain length?

The case in front of me: 98.4% · tirzepatide. Please show the division. I want to check my own against yours. I would like the general form as well as the specific…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
peptide-mapping
peptide-mapping

Enzymatic digestion followed by LC-MS/MS to confirm sequence rather than just mass. The method that catches a scrambled sequence or a D-amino-acid…

103 questions
tirzepatide
tirzepatide

A dual GIP and GLP-1 receptor agonist. Questions here cover the SURPASS and SURMOUNT programmes, the practical differences from a pure GLP-1…

370 questions
asked by stopper_core 28k · 23 months ago
18 votes 5 answers20k views
Does oxidation dominate for liraglutide held at minus 80 °C?

Concretely: oxidation · liraglutide · minus 80 °C. This is one of those things that everyone repeats and nobody derives. This matters practically, not just…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

701 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
liraglutide
liraglutide

A once-daily GLP-1 receptor agonist and the compound that established the class. Still relevant for its shorter half-life, its paediatric and…

235 questions
asked by bufferline42 30k · 2.2 years ago
18 votes5 answers17k views
Does oxidation dominate for liraglutide held at room temperature?

The specifics, since they change the answer: oxidation · liraglutide · room temperature. This is one of those things that everyone repeats and nobody derives. This…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

701 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
liraglutide
liraglutide

A once-daily GLP-1 receptor agonist and the compound that established the class. Still relevant for its shorter half-life, its paediatric and…

235 questions
asked by day_seven_trough 6.7k · 22 months ago
18 votes4 answers15k views
Does aggregation dominate for cagrilintide held at minus 80 °C?

Setup, so nobody has to ask: aggregation · cagrilintide · minus 80 °C. I keep seeing this stated as a fact with no explanation attached, and unexplained facts make me…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

701 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
amylin
amylin

Amylin and its analogues, most prominently cagrilintide, as a satiety mechanism orthogonal to incretin signalling. Includes the pharmacology of…

237 questions
asked by valentina_rossi 9.7k · 12 months ago
18 votes 5 answers14k views
Why would Janoshik report 98.4% where the supplier certificate says something higher?

What I am working with: Janoshik · 98.4%. I noticed this today and I have not touched anything since, in case the state is diagnostic. I have not discarded anything…

purity
purity

Purity as chromatographic area per cent - the fraction of detected material that is your target peak. It says nothing about how much material is…

833 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
batch-testing
batch-testing

Testing at the batch or lot level: sampling plans, how many vials from a lot need testing to say anything about the lot, and the difference…

910 questions
janoshik
janoshik

Janoshik Analytical, an independent third-party testing laboratory widely used by this community for HPLC purity, identity and quantified content…

139 questions
asked by void_volume 9.5k · 6 months ago
18 votes 5 answers10k views
Does aspartimide formation dominate for tirzepatide held at minus 80 °C?

Concretely: aspartimide formation · tirzepatide · minus 80 °C. I want to know whether this is a real physical effect or an artefact of how it is measured. What…

peptide-stability
peptide-stability

The chemistry of peptide degradation: deamidation, oxidation, hydrolysis, aggregation and fibrillation, and how temperature, pH, ionic strength,…

908 questions
storage
storage

Storage conditions and their evidence base: minus twenty degrees for powder, refrigerated for solution, protection from light, and what the…

701 questions
hplc
hplc

Reverse-phase high-performance liquid chromatography, the workhorse purity method. Column chemistry, mobile-phase choice, gradient slope,…

503 questions
tirzepatide
tirzepatide

A dual GIP and GLP-1 receptor agonist. Questions here cover the SURPASS and SURMOUNT programmes, the practical differences from a pure GLP-1…

370 questions
asked by kelvin_lam 7.6k · 4 months ago