Page 9 of 21 · 503 questions tagged hplc.
The method section is present, which is unusual enough that I want to make use of it. I suspect the honest answer is that it depends, in which case I would like to…
The particulars: dulaglutide · Medutest. I have read the primary source rather than the summary, which has left me with more questions. I understand the headline. I…
The method section is present, which is unusual enough that I want to make use of it. I would like to know the limits of what can be inferred from this. What I am…
Setup, so nobody has to ask: QST · an HPLC purity test. I want the working, not the result — I need to be able to redo it with different numbers. I care about the…
Conditions: 98.2% · method section. The figures are clear enough; the question is what they mean and what they do not. I can supply the numbers if the specifics…
Conditions: PeptideMeter · 98.4%. I think I have a problem. I am not yet sure whether it is a real problem or a measurement artefact. I want to know whether this is…
The method section is present, which is unusual enough that I want to make use of it. I want to know whether this is a real physical effect or an artefact of how it…
The particulars: fibrillation · ecnoglutide · 2–8 °C. I understand the observation; what I do not understand is the mechanism behind it. I have read the two review…
The particulars: mazdutide · Janoshik. This is presented as though it settles something, and I am not convinced it does. I have two documents that appear to disagree,…
What I have: 97.1% · orforglipron. This should be a straightforward calculation and I keep getting two different answers. The numbers are arbitrary; the method is…
Conditions: 97.1% · method section. The figures are clear enough; the question is what they mean and what they do not. I can supply the numbers if the specifics…
The particulars: oral semaglutide · a wide-pore C18 column · tirzepatide. The comparison I want does not seem to exist anywhere in a form I can evaluate. I have read…
The case in front of me: 98.2% · liraglutide. I want the working, not the result — I need to be able to redo it with different numbers. I care about the precision as…
A report I have includes traces at two wavelengths from the same injection, using a diode-array detector. The 214 nm trace integrates to 98.6% and the 280 nm trace to…
The vial was lyophilised at the time, which I understand may matter a great deal. I keep seeing this stated as a fact with no explanation attached, and unexplained…
Conditions: PeptideMeter · 97.1%. I think I have a problem. I am not yet sure whether it is a real problem or a measurement artefact. I want to know whether this is…
The case in front of me: oxidation · tirzepatide · 25 °C. This is one of those things that everyone repeats and nobody derives. This matters practically, not just…
What I have: 96.4% · a C8 column · tirzepatide. This is presented as though it settles something, and I am not convinced it does. I have two documents that appear to…
For reference: 97.1% · ecnoglutide. I would rather understand the derivation than memorise the outcome. Two people I asked gave two answers that differ by a factor of…
Numbers first: 97.6% · lot-specific certificate. This is presented as though it settles something, and I am not convinced it does. I have two documents that appear to…
What I have: mazdutide · a C8 column · tirzepatide. I want to know what the trade-off actually is rather than which option is fashionable. I would rather have a…
Details up front: Medutest · 97.1%. I have a result I cannot explain, and I would rather diagnose it than guess. I have checked the obvious explanations and…
Setup, so nobody has to ask: cagrilintide · Janoshik. I can parse the result. I am less sure what it licenses me to conclude. I have deliberately not looked at anyone…
The particulars: oral semaglutide · a wide-pore C18 column · mazdutide. I am trying to choose between two options that are usually discussed as though only one…